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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using GLIS1 Polyclonal antibody (23138-1-AP)

    WB analysis of Jurkat using 23138-1-AP (same clone as 23138-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23138-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using GLIS1 Polyclonal antibody (23138-1-AP)

    WB analysis of Jurkat using 23138-1-AP (same clone as 23138-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23138-1-AP (GLIS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLIS1 Polyclonal antibody (23138-1-AP)

    IHC staining of mouse ovary using 23138-1-AP (same clone as 23138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23138-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using GLIS1 Polyclonal antibody (23138-1-AP)

    IHC staining of mouse ovary using 23138-1-AP (same clone as 23138-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 23138-1-AP (GLIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23138-1-PBS in a different storage buffer formulation.

GLIS1 Polyclonal antibody, PBS Only

GLIS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 23138-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

GLI similar 1, GLIS family zinc finger 1, GLI-similar 1, Zinc finger protein GLIS1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23138-1-PBS targets GLIS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19214

Product name: Recombinant human GLIS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 360-620 aa of BC101799

Sequence: GPDTEADVLTECLVLQQLHTSTQLAASDGKGGCGLGQELLPGVYPGSITPHNGLASGLLPPAHDVPSRHHPLDATTSSHHHLSPLPMAESTRDGLGPGLLSPIVSPLKGLGPPPLPPSSQSHSPGGQPFPTLPSKPSYPPFQSPPPPPLPSPQGYQGSFHSIQSCFPYGDCYRMAEPAAGGDGLVGETHGFNPLRPNGYHSLSTPLPATGYEALAEASCPTALPQQPSEDVVSSGPEDCGFFPNGAFDHCLGHIPSIYTDT

相同性解析による交差性が予測される生物種
Full Name GLIS family zinc finger 1
Calculated molecular weight 620 aa, 66 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC101799
Gene Symbol GLIS1
Gene ID (NCBI) 148979
RRIDAB_2879216
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8NBF1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GLIS1 is a nuclear zinc finger transcription factor that integrates developmental signaling and transcriptional control during neural and epithelial differentiation. It enhances cellular reprogramming efficiency and modulates pathways linked to Hedgehog and Wnt signaling. Dysregulated GLIS1 expression contributes to cardiac malformations, cancer progression, and stemness reactivation, highlighting its dual role in developmental regulation and disease pathogenesis.

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