Jurkat cells were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 16551-1-AP (GLI4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed A549 cells using GLI4 antibody (16551-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 16551-1-PBS in a different storage buffer formulation.