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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLI2-Specific Polyclonal antibody (18989-1-AP)

    WB analysis using 18989-1-AP (same clone as 18989-1-PBS)

    Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GLI2-Specific Polyclonal antibody (18989-1-AP)

    WB analysis of HEK-293 using 18989-1-AP (same clone as 18989-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using GLI2-Specific Polyclonal antibody (18989-1-AP)

    WB analysis of human testis using 18989-1-AP (same clone as 18989-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 18989-1-AP (GLI2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GLI2-Specific Polyclonal antibody (18989-1-AP)

    IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GLI2-Specific Polyclonal antibody (18989-1-AP)

    IHC staining of human kidney using 18989-1-AP (same clone as 18989-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18989-1-AP (GLI2-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GLI2-Specific Polyclonal antibody (18989-1-AP)

    IF Staining of HepG2 using 18989-1-AP (same clone as 18989-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 18989-1-AP (GLI2-Specific antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GLI2-Specific Polyclonal antibody (18989-1-AP)

    IF Staining of MCF-7 using 18989-1-AP (same clone as 18989-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using Gli2 antibody 18989-1-AP at 1:25 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green). This data was developed using the same antibody clone with 18989-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GLI2-Specific Polyclonal antibody, PBS Only

GLI2-Specific Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18989-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Gli2, Thp, Tax helper protein, GLI Kruppel family member GLI2, Gli 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18989-1-PBS targets GLI2-Specific in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name GLI family zinc finger 2
Calculated molecular weight 168 kDa
Observed molecular weight167 kDa， 88 kDa
GenBank accession numberNM_005270
Gene Symbol GLI2
Gene ID (NCBI) 14633
RRIDAB_10596479
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10070
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GLI2, also named as THP, belongs to the GLI C2H2-type zinc-finger protein family. GLI2 play a role during embryogenesis. It binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element that augments the Tax-dependent enhancer of human T-cell leukemia virus type 1. It is implicated in the transduction of SHH signal. Mutation of GLI2 will cause of holoprosencephaly type 9 (HPE9). Four possible isoforms (hGli2a, b, g, and d) are formed by combinations of two independent alternative splicings, and all the isoforms could bind to a DNA motif, TRE2S. hGli2 a, b, g, and d. These isoforms encode 133-, 131-, 88-, and 86-kDa proteins, respectively.

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