Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, U2OS cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
28245-1-AP targets GLI2-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28422 Product name: Recombinant human GLI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 601-795 aa of NM_005270 Sequence: NDVHLRTPLLKENGDSEAGTEPGGPESTEASSTSQAVEDCLHVRAIKTESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGVEMPGTGPGSLGDLTALDDTPPGADTSALAAPSAGGLQLRKHMTTMHRFEQLKKEKLKSLKDSCSWAGPTPHTRNTKLPPLPGSGSILENFSGSGGGGPAGLLPNPRLSELSASE 相同性解析による交差性が予測される生物種 |
| Full Name | GLI family zinc finger 2 |
| Calculated molecular weight | 168 aa |
| Observed molecular weight | 180-200 kDa |
| GenBank accession number | NM_005270 |
| Gene Symbol | GLI2 |
| Gene ID (NCBI) | 2736 |
| RRID | AB_3086041 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLI2, along with the related GLI1 and GLI3, is a member of the GLI family of transcription factors belonging to the Glis superfamily. Glioma-Associated Oncogene Family Zinc Finger 2 (GLI2) is a zinc-finger transcription factor involved in the Sonic Hedgehog pathway. GLI2 is involved in proliferation, invasion, fibrosis, and is involved in carcinogenesis in various malignancies including gastric cancer, lung cancer, prostate cancer, bladder cancer, and so on.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GLI2-Specific antibody 28245-1-AP | Download protocol |
| WB protocol for GLI2-Specific antibody 28245-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |