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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLG1 Polyclonal antibody (34250-1-AP)

    WB analysis using 34250-1-AP (same clone as 34250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34250-1-AP (GLG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GLG1 Polyclonal antibody (34250-1-AP)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GLG1 Polyclonal antibody (34250-1-AP)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using GLG1 Polyclonal antibody (34250-1-AP)

    FC experiment of HepG2 using 34250-1-AP (same clone as 34250-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.5 ug GLG1 Polyclonal antibody (34250-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

GLG1 Polyclonal antibody, PBS Only

GLG1 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 34250-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

CFR 1, CFR1, E selectin ligand 1, ESL 1, ESL1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

34250-1-PBS targets GLG1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag41864

Product name: Recombinant human GLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 997-1146 aa of BC060822

Sequence: LDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNY

相同性解析による交差性が予測される生物種
Full Name golgi apparatus protein 1
Calculated molecular weight 135 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC060822
Gene Symbol GLG1
Gene ID (NCBI) 2734
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ92896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi apparatus protein 1 (GLG1) is also named CFR1, E-selectin ligand 1 (ESL-1) and MG160. GLG1 is a 150-160 kDa membrane sialoglycoprotein, originally isolated from the Golgi apparatus of rat neurons. Two homologs, the chicken cysteine-rich fibroblast growth factor receptor (CFR) and ESL-1, were identified in embryonic chick and murine myeloid cells, respectively (PMID: 35543162). GLG1 was originally identified as an FGF2 receptor, found in the Golgi complex (PMID:7531823). GLG1, a non-tyrosine kinase FGF receptor, is also regulated by RNA splicing (PMID: 7768993). GLG1 was found due to its significant upregulation in GC and correlation with advanced stages and lymph node metastases (PMID: 41287415).

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