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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GLG1 Polyclonal antibody (34250-1-AP)

    WB analysis using 34250-1-AP (same clone as 34250-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34250-1-AP (GLG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GLG1 Polyclonal antibody (34250-1-AP)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GLG1 Polyclonal antibody (34250-1-AP)

    IHC staining of mouse brain using 34250-1-AP (same clone as 34250-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 34250-1-AP (GLG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using GLG1 Polyclonal antibody (34250-1-AP)

    FC experiment of HepG2 using 34250-1-AP (same clone as 34250-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.5 ug GLG1 Polyclonal antibody (34250-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 34250-1-PBS in a different storage buffer formulation.

GLG1 Polyclonal antibody, PBS Only

GLG1 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 34250-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

CFR 1, CFR1, E selectin ligand 1, ESL 1, ESL1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

34250-1-PBS targets GLG1 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag41864

Product name: Recombinant human GLG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 997-1146 aa of BC060822

Sequence: LDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNY

相同性解析による交差性が予測される生物種
Full Name golgi apparatus protein 1
Calculated molecular weight 135 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC060822
Gene Symbol GLG1
Gene ID (NCBI) 2734
RRIDAB_3743180
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ92896
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Golgi apparatus protein 1 (GLG1) is also named CFR1, E-selectin ligand 1 (ESL-1) and MG160. GLG1 is a 150-160 kDa membrane sialoglycoprotein, originally isolated from the Golgi apparatus of rat neurons. Two homologs, the chicken cysteine-rich fibroblast growth factor receptor (CFR) and ESL-1, were identified in embryonic chick and murine myeloid cells, respectively (PMID: 35543162). GLG1 was originally identified as an FGF2 receptor, found in the Golgi complex (PMID:7531823). GLG1, a non-tyrosine kinase FGF receptor, is also regulated by RNA splicing (PMID: 7768993). GLG1 was found due to its significant upregulation in GC and correlation with advanced stages and lymph node metastases (PMID: 41287415).

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