Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33606-1-AP targets GLCE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag35424 Product name: Recombinant human GLCE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 29-200 aa of BC160046 Sequence: NKCSSDKAIQFPRRSSSGFRVDGFEKRAAASESNNYMNHVAKQQSEEAFPQEQQKAPPVVGGFNSNVGSKVLGLKYEEIDCLINDEHTIKGRREGNEVFLPFTWVEKYFDVYGKVVQYDGYDRFEFSHSYSKVYAQRAPYHPDGVFMSFEGYNVEVRDRVKCISGVEGVPLS 相同性解析による交差性が予測される生物種 |
| Full Name | glucuronic acid epimerase |
| Observed molecular weight | 70 kDa |
| GenBank accession number | BC160046 |
| Gene Symbol | GLCE |
| Gene ID (NCBI) | 26035 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O94923 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GLCE encodes the C5-epimerase responsible for converting glucuronic acid to iduronic acid during heparan sulfate biosynthesis. Localized in the Golgi apparatus, it indirectly regulates growth factor signaling, angiogenesis, and cell-cell interactions through structural diversification of glycosaminoglycan chains. Dysregulation of GLCE has been associated with cancer progression and developmental abnormalities, highlighting its role at the interface of glycobiology and disease. (PMID: 24499487)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GLCE antibody 33606-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |

