Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, C6 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
| Positive FC (Intra) detected in | Neuro-2a cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 28 publications below |
| IHC | See 3 publications below |
| IF | See 15 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
20785-1-AP targets GLAST/EAAT1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, cow |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14177 Product name: Recombinant human SLC1A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 471-542 aa of BC037310 Sequence: VDWFLDRLRTTTNVLGDSLGAGIVEHLSRHELKNRDVEMGNSVIEENEMKKPYQLIAQDNETEKPIDSETKM 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 1 (glial high affinity glutamate transporter), member 3 |
| Calculated molecular weight | 542 aa, 60 kDa |
| Observed molecular weight | 50-55 kDa, 90-100 kDa |
| GenBank accession number | BC037310 |
| Gene Symbol | GLAST |
| Gene ID (NCBI) | 6507 |
| RRID | AB_2878738 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43003 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SLC1A3, also known as EAAT-1 or GLAST, is a membrane-bound protein localized in glial cells and pre-synaptic glutamatergic nerve endings. It transports the excitatory neurotransmitters L-glutamate and D-aspartate, which is essential for terminating the postsynaptic acction of glutamate. Recently, a correlation between expression/function of glial EAAT-1 and tumor proliferation has been reported. The exceptionally rare expression of EAAT-1 in non-neoplastic choroid plexus (CP) compared to choroid plexus tumors (CPT) may distinguishes neoplastic from normal CP. There are a number of splicing variants of SLC1A3, like GLAST1a and GLAST1b, exist due to the exon skipping. It also undergo glycosylation. Variety of bands can be observed in the western blotting assay: 50-55 kDa represents the unglycosylated GLAST1a or GLAST1b, 65-70 kDa correspond to the glycosylated proteins, larger proteins between 90-130 kDa may be the multimers of SLC1A3. (11086157, 17471058, 12546822)
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| IF protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| IP protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| WB protocol for GLAST/EAAT1 antibody 20785-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Metabolic regulation of the glioblastoma stem cell epitranscriptome by malate dehydrogenase 2 | ||
Cell Stem Cell Molecular signature of primate astrocytes reveals pathways and regulatory changes contributing to human brain evolution | ||
Nat Commun Major urinary protein 1 modulates neuronal excitability in the medial prefrontal cortex in a male mouse model of affective empathy. | ||
J Adv Res SA supplementation during lactation promotes learning and memory by reducing H3K27me3 levels | ||
Mol Cell Functional nutrient-genetic profiling reveals biotin and FBXW7 are essential to bypass glutamine addiction | ||
Nucleic Acids Res H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor. |