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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig

    WB result of GLA antibody (66121-1-Ig, 1:2000) with si-Control and si-GLA transfected HEK293 cells..

  • Western Blot (WB) analysis of various lysates using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis using 66121-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HeLa cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HeLa using 66121-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HepG2 using 66121-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of HEK-293 using 66121-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of human kidney using 66121-1-Ig

    human kidney tissue were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    WB analysis of LNCaP using 66121-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human kidney using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IHC staining of human liver using 66121-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66121-1-Ig (Alpha galactosidase A antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66121-1-Ig (Alpha galactosidase A antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Alpha Galactosidase A Monoclonal antibody (66121-1-Ig)

    IF Staining of HepG2 using 66121-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Alpha Galactosidase A antibody (66121-1-Ig, Clone: 2B2C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Featured Product
  • KD/KO Validated

Alpha Galactosidase A Monoclonal antibody

Alpha Galactosidase A Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66121-1-Ig
Clone No.2B2C5
Publications(2)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GLA, 2B2C5, Alpha-D-galactosidase A, Alpha-D-galactoside galactohydrolase, Alpha-galactosidase A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, human kidney tissue, LNCaP cells, A431 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66121-1-Ig targets Alpha Galactosidase A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag7505

Product name: Recombinant human GLA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 81-425 aa of BC002689

Sequence: WKDAGYEYLCIDDCWMAPQRDSEGRLQADPQRFPHGIRQLANYVHSKGLKLGIYADVGNKTCAGFPGSFGYYDIDAQTFADWGVDLLKFDGCYCDSLENLADGYKHMSLALNRTGRSIVYSCEWPLYMWPFQKPNYTEIRQYCNHWRNFADIDDSWKSIKSILDWTSFNQERIVDVAGPGGWNDPDMLVIGNFGLSWNQQVTQMALWAIMAAPLFMSNDLRHISPQAKALLQDKDVIAINQDPLGKQGYQLRQGDNFEVWERPLSGLAWAVAMINRQEIGGPRSYTIAVASLGKGVACNPACFITQLLPVKRKLGFYEWTSRLRSHINPTGTVLLQLENTMQMSL

相同性解析による交差性が予測される生物種
Full Name galactosidase, alpha
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC002689
Gene Symbol GLA
Gene ID (NCBI) 2717
RRIDAB_2881520
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06280
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GLA, also named as Melibiase and Alpha-galactosidase A, belongs to the glycosyl hydrolase 27 family. It hydrolyzes terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. Fabry disease is an X-linked lysosomal storage disorder resulting from the deficient activity of GLA. Enzyme replacement therapy (ERT) with GLA is currently the most effective therapeutic strategy for patients with Fabry disease, a lysosomal storage disease.

Protocols

Product Specific Protocols
IF protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
IHC protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
WB protocol for Alpha Galactosidase A antibody 66121-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

bioRxiv

Antigen-display exosomes provide adjuvant-free protection against SARS-CoV-2 disease at nanogram levels of spike protein

Authors - Chenxu Guo
View Article
humanWB

Nat Commun

Deficiency of the frontotemporal dementia gene GRN results in gangliosidosis

Authors - Sebastian Boland
View Article
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