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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GK3P Polyclonal antibody (14176-1-AP)

    WB analysis of HeLa using 14176-1-AP (same clone as 14176-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using GK3P Polyclonal antibody (14176-1-AP)

    WB analysis of mouse testis using 14176-1-AP (same clone as 14176-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14176-1-AP (GK3P antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GK3P Polyclonal antibody (14176-1-AP)

    IHC staining of human skin cancer using 14176-1-AP (same clone as 14176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using GK3P Polyclonal antibody (14176-1-AP)

    IHC staining of human skin cancer using 14176-1-AP (same clone as 14176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer using 14176-1-AP (GK3P antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14176-1-PBS in a different storage buffer formulation.

GK3P Polyclonal antibody, PBS Only

GK3P Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 14176-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14176-1-PBS targets GK3P in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5383

Product name: Recombinant human GK3P protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 208-553 aa of BC066960

Sequence: FNIHSLEWDKQLCEFFGIPMEILPHVRSSSEIYGLMKAGALEGVPISGCLGDQSAALVGQMCFQIGQAKNTYGTGCFLLCNTGHKCVFSDHGLLTTVAYKLGRDKPVYYALEGSVAIAGAVIRWLRDNLGIIKTSEEIEKLAKEVGTSYGCYFVPAFSGLYAPYWEPSARGIICGLTQFTNKCHIAFAALEAVCFQTREILDAMNRDCGIPLSHLQVDGGMTSNKILMQLQADILYIPVVKPLMPETTALGAAMAAGAAEGVDVWSLEPEDLSAVTMKRFEPQINAEESEIRYSTWKKAVMKSMGWVTTQSPEGGDPSVFCSLPLGFFIVSSMAMLIGARYISGIP

相同性解析による交差性が予測される生物種
Full Name glycerol kinase 3 pseudogene
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC066960
Gene Symbol GK3P
Gene ID (NCBI) 2713
RRIDAB_1288669
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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