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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GJC2 Polyclonal antibody

GJC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55376-1-AP

Synonyms

Connexin 46.6, Connexin 47, CX46.6, Cx47, Gap junction alpha 12 protein, Gap junction gamma 2 protein, GJA12, GJC2, HLD2, PMLDAR, SPG44

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 55376-1-AP

    WB analysis of mouse brain using 55376-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55376-1-AP (GJC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human cerebellum using 55376-1-AP

    IHC staining of human cerebellum using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 55376-1-AP

    IHC staining of human brain using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 55376-1-AP

    IHC staining of human brain using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human cerebellum using 55376-1-AP

    IHC staining of human cerebellum using 55376-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 55376-1-AP (GJC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55376-1-AP targets GJC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name gap junction protein, gamma 2, 47kDa
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_020435
Gene symbol GJC2
Gene ID (NCBI) 57165
RRIDAB_2881317
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GJC2 antibody 55376-1-APDownload protocol
IHC protocol for GJC2 antibody 55376-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols