Validation Data Gallery
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Tested Applications
| Positive IP detected in | mouse skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27166-1-AP targets GJB5 in IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26052 Product name: Recombinant human GJB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 211-273 aa of BC004379 Sequence: KRCHECLAARKAQAMCTGHHPHGTTSSCKQDDLLSGDLIFLGSDSHPPLLPDRPRDHVKKTIL 相同性解析による交差性が予測される生物種 |
| Full Name | gap junction protein, beta 5, 31.1kDa |
| Calculated molecular weight | 31 kDa |
| Observed molecular weight | 28 kDa |
| GenBank accession number | BC004379 |
| Gene Symbol | GJB5 |
| Gene ID (NCBI) | 2709 |
| RRID | AB_3085930 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95377 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GJB5, also known as Connexin31.1, is a connexin subtype associated with apoptosis in organs such as the eye and ovary; it is not highly expressed in many tissues in normal conditions (PMID:32592185).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GJB5 antibody 27166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |