Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | A549 cells, K-562 cells, HSC-T6 cells, HEK-293 cells, HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67858-1-Ig targets GIT2 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.
| Tested Reactivity | Human, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0321 Product name: Recombinant human GIT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 254-462 aa of BC001379 Sequence: DSSLDLSELAKAAKKKLQSLSNHLFEELAMDMYDEVDRRETDAVWLATQNHSALVTETTVVPFLPVNPEYSSTRNQGRQKLARFNAHEFATLVIDILSDAKRRQQGSSLSGSKDNVELILKTINNQHSVESQDNDQPDYDSVASDEDTDLETTASKTNRQKSLDSDLSDGPVTVQEFMEVKNALVASEAKIQQLMKVNNNLSDELRIMQ 相同性解析による交差性が予測される生物種 |
| Full Name | G protein-coupled receptor kinase interacting ArfGAP 2 |
| Calculated molecular weight | 95 kDa |
| Observed molecular weight | 70-85 kDa |
| GenBank accession number | BC001379 |
| Gene Symbol | GIT2 |
| Gene ID (NCBI) | 9815 |
| RRID | AB_2918616 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14161 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GIT2 antibody 67858-1-Ig | Download protocol |
| WB protocol for GIT2 antibody 67858-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |