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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GIPR Polyclonal antibody

GIPR Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28322-1-AP

Synonyms

GIP R, GIPR

Validation Data Gallery

Applications:
  • WB analysis using 28322-1-AP

    WB analysis using 28322-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 28322-1-AP (GIPR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis using 28322-1-AP

    WB analysis using 28322-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 28322-1-AP (GIPR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28322-1-AP

    IHC staining of mouse brain using 28322-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28322-1-AP (GIPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28322-1-AP

    IHC staining of mouse brain using 28322-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28322-1-AP (GIPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-1080 cells, K-562 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

28322-1-AP targets GIPR in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIPR fusion protein Ag28143 相同性解析による交差性が予測される生物種
Full Name gastric inhibitory polypeptide receptor
Calculated molecular weight 466 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC093723
Gene symbol GIPR
Gene ID (NCBI) 2696
RRIDAB_2881113
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GIPR antibody 28322-1-APDownload protocol
IHC protocol for GIPR antibody 28322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

Gut-derived peptide hormone receptor expression in the developing mouse hypothalamus

Authors - Lídia Cantacorps
View Article