Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
15651-1-AP targets GINS3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8247 Product name: Recombinant human GINS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC005879 Sequence: MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAVPQGSKLELPLWLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQKGLNDFQCWEKGQASQITASNLVQNYKKRKFTDMED 相同性解析による交差性が予測される生物種 |
| Full Name | GINS complex subunit 3 (Psf3 homolog) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC005879 |
| Gene Symbol | GINS3 |
| Gene ID (NCBI) | 64785 |
| RRID | AB_2247477 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GINS3 (GINS Complex Subunit 3), also known as PSF3, serves as a subunit of the GINS heterotetrameric complex (GINS1/2/3/4). GINS3 is essential for the initiation of DNA replication and progression of replication forks. GINS3 is significantly upregulated in liver hepatocellular carcinoma, where it correlates with poor prognosis, methylation alterations, and immune escape, and has been proposed as a potential diagnostic biomarker.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GINS3 antibody 15651-1-AP | Download protocol |
| WB protocol for GINS3 antibody 15651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Nuclear compartmentalization at the G1/S transition plays a key role in DNA replication control. | ||
Mol Cell Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. | ||
J Clin Invest Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. | ||
Cell Rep Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors | ||
JCI Insight Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia |