Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
15651-1-AP targets GINS3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8247 Product name: Recombinant human GINS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC005879 Sequence: MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAVPQGSKLELPLWLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFGSQLLHFDSPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQKGLNDFQCWEKGQASQITASNLVQNYKKRKFTDMED 相同性解析による交差性が予測される生物種 |
| Full Name | GINS complex subunit 3 (Psf3 homolog) |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC005879 |
| Gene Symbol | GINS3 |
| Gene ID (NCBI) | 64785 |
| RRID | AB_2247477 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BRX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for GINS3 antibody 15651-1-AP | Download protocol |
| WB protocol for GINS3 antibody 15651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis. | ||
J Clin Invest Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. | ||
JCI Insight Partial loss-of-function mutations in GINS4 lead to NK cell deficiency with neutropenia | ||
Cell Rep Loss of POLE3-POLE4 unleashes replicative gap accumulation upon treatment with PARP inhibitors |
