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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GINS1 Polyclonal antibody (16061-1-AP)

    WB analysis using 16061-1-AP (same clone as 16061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16061-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using GINS1 Polyclonal antibody (16061-1-AP)

    WB analysis using 16061-1-AP (same clone as 16061-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16061-1-AP (GINS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16061-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using GINS1 Polyclonal antibody (16061-1-AP)

    IHC staining of human ovary cancer using 16061-1-AP (same clone as 16061-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16061-1-AP (GINS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16061-1-PBS in a different storage buffer formulation.

GINS1 Polyclonal antibody, PBS Only

GINS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 16061-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

PSF1, KIAA0186, GINS complex subunit 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16061-1-PBS targets GINS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8895

Product name: Recombinant human GINS1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-196 aa of BC012542

Sequence: MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIPTIKFRHCSLLRNRRCTVAYLYDRLLRIRALRWEYGSILPNALRFHMAAEEMEWFNNYKRSLATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGEFEVDDGTSVLLKKNSQHFLPRWKCEQLIRQGVLEHILS

相同性解析による交差性が予測される生物種
Full Name GINS complex subunit 1 (Psf1 homolog)
Calculated molecular weight 196 aa, 23 kDa
Observed molecular weight20 kDa
GenBank accession numberBC012542
Gene Symbol GINS1
Gene ID (NCBI) 9837
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14691
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GINS1, also known as Partner of SLD Five 1 (PSF1) is a member of the GINS (Go-Ichi-Nii-San) complex, which plays a vital role in the initiation and elongation of DNA replication (PMID: 34414190). GINS1 was initially found to be predominantly expressed in highly proliferating cells instead of mature cells. Later on, it was found that GINS was involved in the tumorigenesis of various cancers, including breast cancer, colorectal cancer, and hepatocellular carcinoma (PMID: 36065190).

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