Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33593-1-AP targets GIMAP6 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38704 Product name: Recombinant human GIMAP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 38-240 aa of NM_024711.5 Sequence: PRRLRLILMGKTGSGKSATGNSILGRDVFESKLSTRPVTKTSQRRSREWAGKELEVIDTPNILSPQVSPEVADAICQAIVLSAPGPHAVLLVTQLGRFTDEDQQVVRRLQEVFGVGVLGHTILVFTRKEDLAGGSLEDYVRETNNQALAWLDVTLARRHCGFNNRAQGEEQEAQLRELMEKVEAIMWENEGDYYSNKAYQYTQ 相同性解析による交差性が予測される生物種 |
| Full Name | GTPase, IMAP family member 6 |
| Calculated molecular weight | 33kDa，292aa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | NM_024711.5 |
| Gene Symbol | GIMAP6 |
| Gene ID (NCBI) | 474344 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6P9H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GTPase of immunity associated protein 6 (GIMAP6) is a member of the GIMAP family of small GTPases predominantly expressed in lymphoid tissues. GIMAP6 plays a critical role in regulating immune cell survival, particularly in T and B lymphocytes. It localizes to the endoplasmic reticulum and mitochondria, where it modulates autophagy and apoptosis pathways. Studies indicate that GIMAP6 deficiency leads to spontaneous apoptosis of lymphocytes, highlighting its essential function in maintaining immune homeostasis. Additionally, GIMAP6 has been implicated in autoimmune diseases and certain hematological malignancies, making it an emerging therapeutic target for modulating immune responses and treating lymphocyte-related disorders (PMID: 35551368; 33328581; 29718959).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GIMAP6 antibody 33593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |