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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GIF Polyclonal antibody (27474-1-AP)

    WB analysis using 27474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27474-1-AP (GIF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

GIF Polyclonal antibody

GIF Polyclonal Antibody for WB, ELISA
Cat No. 27474-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Intrinsic factor, INF, IFMH, IF, Gastric intrinsic factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27474-1-AP targets GIF in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIF fusion protein Ag26500 相同性解析による交差性が予測される生物種
Full Name gastric intrinsic factor (vitamin B synthesis)
Calculated molecular weight 45 kDa
Observed molecular weight45 kDa
GenBank accession numberBC037958
Gene Symbol GIF
Gene ID (NCBI) 2694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GIF antibody 27474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols