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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using GHITM Polyclonal antibody (16296-1-AP)

    WB analysis of mouse liver using 16296-1-AP (same clone as 16296-1-PBS)

    mouse liver mitochondria tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GHITM Polyclonal antibody (16296-1-AP)

    WB analysis of mouse liver using 16296-1-AP (same clone as 16296-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GHITM Polyclonal antibody (16296-1-AP)

    WB analysis of HEK-293 using 16296-1-AP (same clone as 16296-1-PBS)

    WB results of GHITM antibody (16296-1-AP) with siRNA sample. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using GHITM Polyclonal antibody (16296-1-AP)

    WB analysis of HeLa using 16296-1-AP (same clone as 16296-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using GHITM Polyclonal antibody (16296-1-AP)

    WB analysis of SH-SY5Y using 16296-1-AP (same clone as 16296-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16296-1-AP (GHITM antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GHITM Polyclonal antibody (16296-1-AP)

    IHC staining of mouse brain using 16296-1-AP (same clone as 16296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using GHITM Polyclonal antibody (16296-1-AP)

    IHC staining of mouse brain using 16296-1-AP (same clone as 16296-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16296-1-AP (GHITM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GHITM Polyclonal antibody (16296-1-AP)

    IF Staining of HeLa using 16296-1-AP (same clone as 16296-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GHITM antibody (16296-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16296-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GHITM Polyclonal antibody, PBS Only

GHITM Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16296-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Dermal papilla-derived protein 2, DERP2, Growth hormone-inducible transmembrane protein, MICS1, My021

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16296-1-PBS targets GHITM in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9482

Product name: Recombinant human GHITM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-72 aa of BC010354

Sequence: MLAARLVCLRTLPSRVFHPAFTKASPVVKNSITKNQWLLTPSREYATKTRIGIRRGRTGQELKEAALEPSME

相同性解析による交差性が予測される生物種
Full Name growth hormone inducible transmembrane protein
Calculated molecular weight 345 aa, 37 kDa
Observed molecular weight 42 kDa, 25-27 kDa
GenBank accession numberBC010354
Gene Symbol GHITM
Gene ID (NCBI) 27069
RRIDAB_2111275
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3K2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GHITM, also known as MICS1, TMBIM5 or DERP2, is a mitochondrial protein which localizes in the inner membrane. GHITM is involved in mitochondrial morphology in specific cristae structures and the apoptotic release of cytochrome c from the mitochondria (PMID: 18417609). The gene of GHITM maps to chromosome 10q23.1, and encodes a 345-amino-acid protein with a calculated molecular mass of 37 kDa. The apparent molecular weight has been reported to be 42 kDa, the increased size in the protein may be due to post-translational modifications (PMID: 11416014; 16412389). GHITM can be cleaved into smaller forms of 23-27 kDa (PMID: 16412389; 18417609).

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