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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Growth Hormone Recombinant antibody (82813-2-RR)

    WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

    Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Growth Hormone Recombinant antibody (82813-2-RR)

    WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82813-2-RR (Growth Hormone antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (Growth Hormone antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82813-2-RR

    Affinity and Kinetic Characterization of 82813-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

Growth Hormone Recombinant monoclonal antibody, PBS Only

Growth Hormone Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82813-2-PBS
Clone No.4G8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

GH, GH1, 4G8, GH 1, GH-N

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82813-2-PBS targets Growth Hormone in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0383

Product name: recombinant human GH1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*HIS

Domain: 27-217 aa of BC075012

Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

相同性解析による交差性が予測される生物種
Full Name GH1
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight22 kDa
GenBank accession numberBC075012
Gene Symbol GH1
Gene ID (NCBI) 2688
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01241
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

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