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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

    Human placenta tissue was subjected to SDS PAGE followed by western blot with 82813-2-RR (GH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    WB analysis using 82813-2-RR (same clone as 82813-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82813-2-RR (Growth Hormone antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of human placenta tissue using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    IP experiment of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    IP result of anti-Growth Hormone (IP:82813-2-RR, 4ug; Detection:82813-2-RR 1:5000) with human placenta tissue lysate 960 ug. This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (GH1 antibody) at dilution of 1:2500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Growth Hormone Recombinant monoclonal antibody (82813-2-RR)

    IHC staining of human placenta using 82813-2-RR (same clone as 82813-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82813-2-RR (Growth Hormone antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82813-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82813-2-RR

    Affinity and Kinetic Characterization of 82813-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82813-2-RR against Human GH1 were performed. The affinity constant is 0.76 nM.

Growth Hormone Recombinant monoclonal antibody, PBS Only

Growth Hormone Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82813-2-PBS
Clone No.4G8

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

GH, GH1, GH 1, GH-N, growth hormone 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82813-2-PBS targets Growth Hormone in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0383

Product name: recombinant human GH1 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*HIS

Domain: 27-217 aa of BC075012

Sequence: FPTIPLSRLFDNAMLRAHRLHQLAFDTYQEFEEAYIPKEQKYSFLQNPQTSLCFSESIPTPSNREETQQKSNLELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVETFLRIVQCRSVEGSCGF

相同性解析による交差性が予測される生物種
Full Name GH1
Calculated molecular weight 217 aa, 25 kDa
Observed molecular weight22 kDa
GenBank accession numberBC075012
Gene Symbol GH1
Gene ID (NCBI) 2688
RRIDAB_3086545
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01241
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GH1, also named as GH and GH-N, belongs to the somatotropin/prolactin family. GH1 plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

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