Validation Data Gallery
Tested Applications
| Positive WB detected in | 786-O cells, human urine sample, human milk, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18191-1-AP targets GGTLC2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12911 Product name: Recombinant human GGTLC2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-218 aa of BC069534 Sequence: MTSEFFAAQLRAQISDDTTHPISYYKPEFYTPVDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNDEMDDFSSPNITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQPPSHADHTPMPQAIIYNLWFGYDVKRAVEEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRTAGGWAAASDSRKGGEPAGY 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-glutamyltransferase light chain 2 |
| Calculated molecular weight | 218 aa, 24 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | BC069534 |
| Gene Symbol | GGTLC2 |
| Gene ID (NCBI) | 91227 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14390 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GGT is a protease anchored to the cell membrane, consisting of a light chain and a heavy chain. GGTLC2 (Glutathione hydrolase light chain 2), also known as GGTL4, a constituent of the GGT family, has been characterized as an antioxidant gene, with emerging evidence indicating a potential link between its expression levels and sarcoma patient outcome (PMID: 40191433). Studies have shown that GGTLC2 is highly expressed in gastric cancer, promoting cell proliferation, invasion, and migration while inhibiting apoptosis and ferroptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GGTLC2 antibody 18191-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |