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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GGTLC2 Polyclonal antibody (18191-1-AP)

    WB analysis using 18191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18191-1-AP (GGTLC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GGTLC2 Polyclonal antibody (18191-1-AP)

    WB analysis using 18191-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18191-1-AP (GGTLC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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GGTLC2 Polyclonal antibody

GGTLC2 Polyclonal Antibody for WB, ELISA
Cat No. 18191-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Gamma-glutamyltransferase light chain 2, Gamma-glutamyltransferase-like protein 4, GGTL4, Glutathione hydrolase light chain 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in786-O cells, human urine sample, human milk, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18191-1-AP targets GGTLC2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GGTLC2 fusion protein Ag12911 相同性解析による交差性が予測される生物種
Full Name gamma-glutamyltransferase light chain 2
Calculated molecular weight 218 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC069534
Gene Symbol GGTLC2
Gene ID (NCBI) 91227
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14390
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GGT is a protease anchored to the cell membrane, consisting of a light chain and a heavy chain. GGTLC2 (Glutathione hydrolase light chain 2), also known as GGTL4, a constituent of the GGT family, has been characterized as an antioxidant gene, with emerging evidence indicating a potential link between its expression levels and sarcoma patient outcome (PMID: 40191433). Studies have shown that GGTLC2 is highly expressed in gastric cancer, promoting cell proliferation, invasion, and migration while inhibiting apoptosis and ferroptosis.

Protocols

Product Specific Protocols
WB protocol for GGTLC2 antibody 18191-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols