Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, hTERT-RPE1 cells |
| Positive IHC detected in | human lung cancer tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
Product Information
24674-1-AP targets GGT7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20234 Product name: Recombinant human GGT7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 313-662 aa of BC141843 Sequence: VLDVLGTSGPAAFYAGGNLTLEMVAEAQHAGGVITEEDFSNYSALVEKPVCGVYRGHLVLSPPPPHTGPALISALNILEGFNLTSLVSREQALHWVAETLKIALALASRLGDPVYDSTITESMDDMLSKVEAAYLRGHINDSQAAPAPLLPVYELDGAPTAAQVLIMGPDDFIVAMVSSLNQPFGSGLITPSGILLNSQMLDFSWPNRTANHSAPSLENSVQPGKRPLSFLLPTVVRPAEGLCGTYLALGANGAARGLSGLTQVLLNVLTLNRNLSDSLARGRLHPDLQSNLLQVDSEFTEEEIEFLEARGHHVEKVDVLSWVHGSRRTNNFIIAVKDPRSPDAAGATIL 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-glutamyltransferase 7 |
| Calculated molecular weight | 662 aa, 70 kDa |
| Observed molecular weight | 75-100 kDa |
| GenBank accession number | BC141843 |
| Gene Symbol | GGT7 |
| Gene ID (NCBI) | 2686 |
| RRID | AB_2879667 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJ14 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GGT7 (Glutathione hydrolase 7), a member of the gamma-glutamyltransferase family, is highly expressed in brain and was previously shown to have decreased expression in gliomas. Reports on the protein expression analysis of GGT7 are only limited to ovarian cancer and glioblastoma. Loss of GGT7 would lead to increased ROS activity, and in turn, stimulate tumorigenic phenotypes (PMID: 41316810, 25884624). GGT7 was identified as a heterodimer consisting of two glycosylated subunits with a mean molecular mass of 80 kDa and 29 kDa, respectively (PMID: 33916150).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for GGT7 antibody 24674-1-AP | Download protocol |
| WB protocol for GGT7 antibody 24674-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv Perturbations of glutathione and sphingosine metabolites in Port Wine Birthmark patient-derived induced pluripotent stem cells | ||
Biochem Biophys Res Commun Human PRSS8 functions as a targetable, endogenous TGFBR3 sheddase via the novel cholesterol-PRSS8-TGF-β axis.
| ||
Genome Biol Systematic interrogation of functional genes underlying cholesterol and lipid homeostasis
| ||
Metabolites Perturbations of Glutathione and Sphingosine Metabolites in Port Wine Birthmark Patient-Derived Induced Pluripotent Stem Cells |