Validation Data Gallery
Tested Applications
| Positive WB detected in | 3T3-L1 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30148-1-AP targets GGT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32373 Product name: Recombinant human GGT5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 387-527 aa of BC011362 Sequence: GTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSK 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-glutamyltransferase 5 |
| Calculated molecular weight | 586 aa, 62 kDa |
| Observed molecular weight | 55-60 kDa |
| GenBank accession number | BC011362 |
| Gene Symbol | GGT5 |
| Gene ID (NCBI) | 2687 |
| RRID | AB_3086247 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36269 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Gamma-glutamyltransferase 5 (GGT5) is one of the two GGT family members (GGT1 and GGT5) with catalytic activity identified to date and was first reported in 2008 (PMID:18357469). GGT5 is widely distributed in a variety of tissues, with relatively high expression in liver, kidney, and alveolar macrophages (PMID:25377544). GGT5 played a pivotal role in oxidative regulation, drug metabolism, and immune modulation in the human body (PMID:21447318).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GGT5 antibody 30148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |