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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GGT1 Recombinant antibody (83304-1-RR)

    WB analysis using 83304-1-RR (same clone as 83304-1-PBS)

    Human kidney tissue were subjected to SDS PAGE followed by western blot with 83304-1-RR (GGT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Kidney(renal cell) Cancer using GGT1 Recombinant antibody, PBS Only (83304-1-PBS)

    IHC staining of Human Kidney(renal cell) Cancer using 83304-1-PBS

    Immunohistochemical analysis of paraffin-embedded Human Kidney(renal cell) Cancer slide using 83304-1-PBS (GGT1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GGT1 Recombinant antibody (83304-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using GGT1 Recombinant antibody (83304-1-RR)

    IHC staining of human intrahepatic cholangiocarcinoma using 83304-1-RR (same clone as 83304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83304-1-RR (GGT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83304-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83304-1-PBS

    Affinity and Kinetic Characterization of 83304-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 83304-1-PBS against Human GGT1 was performed. The affinity constant is 1.86 nM.

GGT1 Recombinant monoclonal antibody, PBS Only

GGT1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83304-1-PBS
Clone No.230428B5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

230428B5, CD224, D22S672, D22S732, GGT

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inHuman Kidney(renal cell) Cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83304-1-PBS targets GGT1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34209

Product name: Recombinant human GGT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 381-470 aa of NM_013430

Sequence: TAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVG

相同性解析による交差性が予測される生物種
Full Name gamma-glutamyltransferase 1
Calculated molecular weight61 kDa
Observed molecular weight24 kDa
GenBank accession numberNM_013430
Gene Symbol GGT1
Gene ID (NCBI) 2678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP19440
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GGT1 is a transpeptidase whose activity increases the availability of amino acids, primarily cysteine, for intracellular GSH synthesis. GGT1 also plays a critical role in maintaining GSH homeostasis and defense against oxidative stress. In liver and obstructive biliary diseases, circulating GGT1 activity has widely been used for diagnosis purposes, as well as an indicator of alcohol consumption. (PMID: 31151999)

Protocols

Product Specific Protocols
IHC protocol for GGT1 antibody 83304-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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