Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of mouse brain using 14944-1-AP (same clone as 14944-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 14944-1-AP (same clone as 14944-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 14944-1-AP (same clone as 14944-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
WB analysis of human testis using 14944-1-AP (same clone as 14944-1-PBS)
human testis tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
WB analysis of mouse bladder using 14944-1-AP (same clone as 14944-1-PBS)
mouse bladder tissue were subjected to SDS PAGE followed by western blot with 14944-1-AP (GGPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 14944-1-AP (same clone as 14944-1-PBS)
IP result of anti-GGPS1 (IP:14944-1-AP, 4ug; Detection:14944-1-AP 1:500) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 14944-1-AP (GGPS1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14944-1-AP (GGPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14944-1-AP (same clone as 14944-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 14944-1-AP (GGPS1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14944-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

GGPS1 Polyclonal antibody, PBS Only

GGPS1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14944-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14944-1-PBS targets GGPS1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6745 Product name: Recombinant human GGPS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC067768 Sequence: MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHQDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE 相同性解析による交差性が予測される生物種
Full Name	geranylgeranyl diphosphate synthase 1
Calculated molecular weight	35 kDa
Observed molecular weight	35 kDa
GenBank accession number	BC067768
Gene Symbol	GGPS1
Gene ID (NCBI)	9453
RRID	AB_2110990
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95749
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

GGPS1(Geranylgeranyl pyrophosphate synthase) belongs to the FPP/GGPP synthase family and catalyzes the synthesis of GGPP, an important molecule responsible for the C20-prenylation of protein and for the regulation of a nuclear hormone receptor. GGPP synthase puri¢ed from bovine brain is reported to be a homo-oligomer (150-195 kDa) with a molecular mass of 37.5 kDa for the monomer. This is consistent with the calculated molecular mass (35 kDa) of mouse and human GGPP synthases (PMID:10101267).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 14944-1-AP (same clone as 14944-1-PBS)

WB analysis of human placenta using 14944-1-AP (same clone as 14944-1-PBS)

WB analysis of human heart using 14944-1-AP (same clone as 14944-1-PBS)

WB analysis of human testis using 14944-1-AP (same clone as 14944-1-PBS)

WB analysis of mouse bladder using 14944-1-AP (same clone as 14944-1-PBS)

IP experiment of mouse brain using 14944-1-AP (same clone as 14944-1-PBS)

IHC staining of human kidney using 14944-1-AP (same clone as 14944-1-PBS)