Validation Data Gallery
Tested Applications
| Positive WB detected in | Calu-1 cells, A549 cells, HT-1080 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32984-1-AP targets GGN in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38372 Product name: Recombinant human GGN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-381 aa of NM_152657.3 Sequence: TESQAGPRNQGQTAGRARGGAPPHAGEGEMAQPADSESGLSLLCKITFKSRPSLAPPAASSSLAAKASLGGGGGGGLFAASGAISYAEVLKQGPLPPGAARPLGEVSRGAQEAEGGDGDGEGCSGPPSAPASQARALPPPPYTTFPGSKPKFDWVSAPDGPERHFRFNGAGGGIGAPRRRAA 相同性解析による交差性が予測される生物種 |
| Full Name | gametogenetin |
| Calculated molecular weight | 67kDa，652aa |
| Observed molecular weight | 65 kDa, 70 kDa |
| GenBank accession number | NM_152657.3 |
| Gene Symbol | GGN |
| Gene ID (NCBI) | 199720 |
| RRID | AB_3742813 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q86UU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Human gametogenetin (GGN) is expressed in the testis and ovaries and is a conserved gene in human and mouse. Recently, GGN was found to play a tumor-promoting role in cancer through regulation of NFκB/caspase-3-mediated apoptosis signaling (PMID: 30721393). GGN can be glycosylated, which may result in an increase in observed molecular weight.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GGN antibody 32984-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |