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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of HepG2 using 16209-1-AP (same clone as 16209-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of HepG2 using 16209-1-AP (same clone as 16209-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of PC-3 using 16209-1-AP (same clone as 16209-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of HEK-293 using 16209-1-AP (same clone as 16209-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of MCF-7 using 16209-1-AP (same clone as 16209-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of L02 using 16209-1-AP (same clone as 16209-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using GGCX Polyclonal antibody (16209-1-AP)

    WB analysis of PC-3 using 16209-1-AP (same clone as 16209-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16209-1-AP (GGCX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    IP experiment of mouse liver using 16209-1-AP (same clone as 16209-1-PBS)

    IP result of anti-GGCX (IP:16209-1-AP, 4ug; Detection:16209-1-AP 1:2000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human liver using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human brain using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using GGCX Polyclonal antibody (16209-1-AP)

    IHC staining of human brain using 16209-1-AP (same clone as 16209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 16209-1-AP (GGCX antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16209-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GGCX Polyclonal antibody, PBS Only

GGCX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16209-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16209-1-PBS targets GGCX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9185

Product name: Recombinant human GGCX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 383-758 aa of BC013979

Sequence: LTQGYNNWTNGLYGYSWDMMVHSRSHQHVKITYRDGRTGELGYLNPGVFTQSRRWKDHADMLKQYATCLSRLLPKYNVTEPQIYFDIWVSINDRFQQRIFDPRVDIVQAAWSPFQRTSWVQPLLMDLSPWRAKLQEIKSSLDNHTEVVFIADFPGLHLENFVSEDLGNTSIQLLQGEVTVELVAEQKNQTLREGEKMQLPAGEYHKVYTTSPSPSCYMYVYVNTTELALEQDLAYLQELKEKVENGSETGPLPPELQPLLEGEVKGGPEPTPLVQTFLRRQQRLQEIERRRNTPFHERFFRFLLRKLYVFRRSFLMTCISLRNLILGRPSLEQLAQEVTYANLRPFEAVGELNPSNTDSSHSNPPESNPDPVHSEF

相同性解析による交差性が予測される生物種
Full Name gamma-glutamyl carboxylase
Calculated molecular weight 758 aa, 88 kDa
Observed molecular weight 88 kDa
GenBank accession numberBC013979
Gene Symbol GGCX
Gene ID (NCBI) 2677
RRIDAB_2110874
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP38435
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GGCX(Gamma-glutamyl carboxylase) is also named as GC and belongs to the vitamin K-dependent gamma-carboxylase family. This 94 kDa (including all modifications, such as the five N-linked glycosylations), is a 5-pass transmembrane protein and a key regulator of blood coagulation(PMID:20518534). It mediates the vitamin K-dependent carboxylation of glutamate residues to calcium-binding gamma-carboxyglutamate (Gla) residues with the concomitant conversion of the reduced hydroquinone form of vitamin K to vitamin K epoxide. Defects in GGCX are a cause of combined deficiency of vitamin K-dependent clotting factors type 1 (VKCFD1) and pseudoxanthoma elasticum-like disorder with multiple coagulation factor deficiency (PXEL-MCFD)(PMID:9845520;17110937).

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