Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, U-937 cells, mouse kidney tissue, mouse liver tissue, human brain tissue, HepG2 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | mouse small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
16257-1-AP targets GGCT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9284 Product name: Recombinant human GGCT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-188 aa of BC019243 Sequence: MANSGCKDVTGPDEESFLYFAYGSNLLTERIHLRNPSAAFFCVARLQDFKLDFGNSQGKTSQTWHGGIATIFQSPGDEVWGVVWKMNKSNLNSLDEQEGVKSGMYVVIEVKVATQEGKEITCRSYLMTNYESAPPSPQYKKIICMGAKENGLPLEYQEKLKAIEPNDYTGKVSEEIEDIIKKGETQTL 相同性解析による交差性が予測される生物種 |
| Full Name | gamma-glutamyl cyclotransferase |
| Calculated molecular weight | 188 aa, 21 kDa |
| Observed molecular weight | 21-25 kDa |
| GenBank accession number | BC019243 |
| Gene Symbol | GGCT |
| Gene ID (NCBI) | 79017 |
| RRID | AB_10852962 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75223 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GGCT antibody 16257-1-AP | Download protocol |
| IHC protocol for GGCT antibody 16257-1-AP | Download protocol |
| IF protocol for GGCT antibody 16257-1-AP | Download protocol |
| IP protocol for GGCT antibody 16257-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
FASEB J Taurine treatment prevents derangement of the hepatic γ-glutamyl cycle and methylglyoxal metabolism in a mouse model of classical homocystinuria: regulatory crosstalk between thiol and sulfinic acid metabolism. | ||
Sci Rep Identification of proteins responsible for adriamycin resistance in breast cancer cells using proteomics analysis. | ||
Biochim Biophys Acta Mol Basis Dis Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages | ||
Int J Mol Sci Interaction of MRPL9 and GGCT Promotes Cell Proliferation and Migration by Activating the MAPK/ERK Pathway in Papillary Thyroid Cancer | ||
Redox Biol Unbalanced redox status network as an early pathological event in congenital cataracts | ||
Cancer Sci Myc upregulates Ggct, γ-glutamylcyclotransferase to promote development of p53-deficient osteosarcoma |