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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    WB result of GGA2 antibody (10356-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GGA2 transfected HeLa cells. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse brain using 10356-1-AP (same clone as 10356-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human kidney tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of human kidney using 10356-1-AP (same clone as 10356-1-PBS)

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse adipose tissue using GGA2 Polyclonal antibody (10356-1-AP)

    WB analysis of mouse adipose using 10356-1-AP (same clone as 10356-1-PBS)

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 10356-1-AP (GGA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human colon cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GGA2 Polyclonal antibody (10356-1-AP)

    IHC staining of human stomach cancer using 10356-1-AP (same clone as 10356-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10356-1-AP (GGA2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GGA2 Polyclonal antibody (10356-1-AP)

    IF Staining of HeLa using 10356-1-AP (same clone as 10356-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GGA2 antibody (10356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10356-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GGA2 Polyclonal antibody, PBS Only

GGA2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 10356-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10356-1-PBS targets GGA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0370

Product name: Recombinant human GGA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 70-316 aa of BC000284

Sequence: KIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLGSWATGKVKGRVIEILFSWTVWFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWPKSSIFDADEEKSKLLTRLLKSNHPEDLQAANRLIKNLVKEEQEKSEKVSKRVSAVEEVRSHVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQANDLLTQGVLLYKQVMEG

相同性解析による交差性が予測される生物種
Full Name golgi associated, gamma adaptin ear containing, ARF binding protein 2
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC000284
Gene Symbol GGA2
Gene ID (NCBI) 23062
RRIDAB_2110735
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJY4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GGA2, also known as VEAR, belongs to the GGA protein family and regulates the trafficking of proteins between the trans-Golgi network and the lysosome. GGA2 has an amino-terminal VHS domain in its N terminus which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins.

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