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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using GFPT2 Recombinant monoclonal antibody (86609-3-RR)

    WB analysis of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using GFPT2 Recombinant monoclonal antibody (86609-3-RR)

    WB analysis of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 86609-3-RR (GFPT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A375 cells using GFPT2 Recombinant monoclonal antibody (86609-3-RR)

    IF Staining of A375 using 86609-3-RR (same clone as 86609-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using GFPT2 Recombinant monoclonal antibody (86609-3-RR)

    IF Staining of NIH/3T3 using 86609-3-RR (same clone as 86609-3-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using GFPT2 antibody (86609-3-RR, Clone: 251454E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86609-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86609-3-RR

    Affinity and Kinetic Characterization of 86609-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86609-3-RR against Human GFPT2 were performed. The affinity constant is below 1 pM.

GFPT2 Recombinant monoclonal antibody, PBS Only

GFPT2 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86609-3-PBS
Clone No.251454E5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

D-fructose-6-phosphate amidotransferase 2, EC:2.6.1.16, GFAT2, glutamine fructose 6 phosphate transaminase 2, Glutamine:fructose-6-phosphate amidotransferase 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86609-3-PBS targets GFPT2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7275

Product name: Recombinant human GFPT2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 330-682 aa of BC000012

Sequence: IFEQPESVFNTMRGRVNFETNTVLLGGLKDHLKEIRRCRRLIVIGCGTSYHAAVATRQVLEELTELPVMVELASDFLDRNTPVFRDDVCFFISQSGETADTLLALRYCKDRGALTVGVTNTVGSSISRETDCGVHINAGPEVGVASTKAYTSQFISLVMFGLMMSEDRISLQNRRQEIIRGLRSLPELIKEVLSLEEKIHDLALELYTQRSLLVMGRGYNYATCLEGALKIKEITYMHSEGILAGELKHGPLALIDKQMPVIMVIMKDPCFAKCQNALQQVTARQGRPIILCSKDDTESSKFAYKTIELPHTVDCLQGILSVIPLQLLSFHLAVLRGYDVDFPRNLAKSVTVE

相同性解析による交差性が予測される生物種
Full Name glutamine-fructose-6-phosphate transaminase 2
Calculated molecular weight 77 kDa
Observed molecular weight 75-77 kDa
GenBank accession numberBC000012
Gene Symbol GFPT2
Gene ID (NCBI) 9945
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO94808
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GFPT2 (Glutamine-fructose-6phosphate transaminase 2) is a rate-limiting enzyme in hexosamine biosynthesis involved in the occurrence and progress of many cancers (PMID: 35330716, 14764791). GFPT2 plays an important role in the metabolic activity of cells, especially in their glucose metabolism. GFPT2 expression was highly correlated with EMT-related factors (PMID: 37395335). High GFPT2 expression is associated with poor prognosis in various tumors, including lung adenocarcinoma, colorectal cancer, leiomyosarcoma, and serous ovarian cancer.

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