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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using GFPT1 Polyclonal antibody (14132-1-AP)

    WB analysis of human skeletal muscle using 14132-1-AP (same clone as 14132-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GFPT1 Polyclonal antibody (14132-1-AP)

    WB analysis of HepG2 using 14132-1-AP (same clone as 14132-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14132-1-AP (GFPT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using GFPT1 Polyclonal antibody (14132-1-AP)

    IP experiment of HepG2 using 14132-1-AP (same clone as 14132-1-PBS)

    IP result of anti-GFPT1 (IP:14132-1-AP, 4ug; Detection:14132-1-AP 1:3000) with HepG2 cells lysate 1600 ug. This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GFPT1 Polyclonal antibody (14132-1-AP)

    IHC staining of human gliomas using 14132-1-AP (same clone as 14132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GFPT1 Polyclonal antibody (14132-1-AP)

    IHC staining of human gliomas using 14132-1-AP (same clone as 14132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14132-1-AP (GFPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using GFPT1 Polyclonal antibody (14132-1-AP)

    IHC staining of human hepatocirrhosis using 14132-1-AP (same clone as 14132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using GFPT1 Polyclonal antibody (14132-1-AP)

    IHC staining of human hepatocirrhosis using 14132-1-AP (same clone as 14132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 14132-1-AP (GFPT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GFPT1 Polyclonal antibody (14132-1-AP)

    IF Staining of HepG2 using 14132-1-AP (same clone as 14132-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using GFPT1 antibody 14132-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14132-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GFPT1 Polyclonal antibody, PBS Only

GFPT1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14132-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

D-fructose-6-phosphate amidotransferase 1, EC:2.6.1.16, GFAT, GFAT 1, GFAT1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14132-1-PBS targets GFPT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5312

Product name: Recombinant human GFPT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC045641

Sequence: MCGIFAYLNYHVPRTRREILETLIKGLQRLEYRGYDSAGVGFDGGNDKDWEANACKIQLIKKKGKVKALDEEVHKQQDMDLDIEFDVHLGIAHTRWATHGEPSPVNSHPQRSDKNNEFIVIHNGIITNYKDLKKFLESKGYDFESETDTETIAKLVKYMYDNRESQDTSFTTLVERVIQQLEGAFALVFKSVHFPGQAVGTRRGSPLLIGVRSEHKLSTDHIPILYRTGKDKKGSCNLSRVDSTTCLFPVEEKAVEYYFASDASAVIEHTNRVIFLEDDDVAAVVDGRLSIHRIKRTAGDHPGRAVQTLQMELQQIMKGNFSSFMQKEIFEQPESVVNTMRGRVNFDDYT

相同性解析による交差性が予測される生物種
Full Name glutamine-fructose-6-phosphate transaminase 1
Calculated molecular weight 681 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC045641
Gene Symbol GFPT1
Gene ID (NCBI) 2673
RRIDAB_2110155
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06210
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GFPT1 (glutamine-fructose-6-phosphate transaminase 1) catalyzes the transfer of an amino group from glutamine onto fructose-6-phosphate, yielding glucosamine-6-phosphate (GlcN-6-P) and glutamate. GFPT1 is the key enzyme of the hexosamine pathway and is required for critical events in neuromuscular transmission. Mutations of GFPT1 cause congenital myasthenic syndromes (CMS). (21310273, 25765662)

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