Validation Data Gallery

  • Immunofluorescence Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa488 1:500

    Immunofluorescence Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa488 1:500

  • Western Blot Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa647 1:2,000

    Western Blot Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa647 1:2,000

  • Western Blot Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa647 1:2,000

    Western Blot Primary antibody: Anti-GFP PABG1 1:1,000 Secondary antibody: anti-rabbit_Alexa647 1:2,000

ChromoTek GFP Polyclonal antibody

Rabbit polyclonal antibody to Green Fluorescent Protein
Cat No. pabg1

Host/IsoType

Rabbit / IgG

Specificity

GFP and GFP derivates

Applications

WB, IF/ICC

Conjugate

Unconjugated

GFP, green, green fluorescence protein, EGFP, pHluorin, TagGFP, wtGFP, GFP tag, GFP-tag

Size/Concentration: 

-/ -


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Product Information

Rabbit polyclonal antibody to Green Fluorescent Protein

DescriptionRabbit polyclonal antibody to Green Fluorescent Protein
ApplicationsWB, IF/ICC
Specificity/TargetThe antibody recognizes green fluorescent proteins derived from Aequorea victoria GFP, such as CFP, EGFP, wtGFP, pHluorin (ecliptic), TagGFP, YFP, pHluorin, GFP.
For the complete list, please click here: Fluorescent protein specificity table antibodies
Recommended DilutionWestern blot: 1:1,000
IF/ICC: 1:1,000
Host/IsoTypeRabbit / IgG
ClassPolyclonal
TypeAntibody
ImmunogenGreen Fluorescent Protein (GFP)
UniProtKBP42212
ConjugateUnconjugated
FormLiquid
Purification MethodAntigen affinity purification
RRIDAB_2749857
Storage BufferBuffered aqueous solution. Preservative: 0.09% Sodium Azide
Storage ConditionShipped on blue ice. Store at +4°C short term (1-2 weeks). Aliquot upon delivery. Store at -20°C or -80°C long term. Avoid freeze-thaw cycles. Stable for one year.
Size20 µL; 100 µL
BackgroundGreen Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a rabbit polyclonal antibody against GFP, reactive to wtGFP, EGFP, CFP, TagGFP, YFP, and pHluorin.

Publications

ApplicationTitle
WB

Nature

Equilibrium between nascent and parental MCM proteins protects replicating genomes.

Authors - Hana Sedlackova
IF

Nat Commun

SPRTN patient variants cause global-genome DNA-protein crosslink repair defects

Authors - Pedro Weickert
IF

Nat Commun

TRAIP resolves DNA replication-transcription conflicts during the S-phase of unperturbed cells

Authors - Shaun Scaramuzza

Mol Cell

RNF14-dependent atypical ubiquitylation promotes translation-coupled resolution of RNA-protein crosslinks

Authors - Shubo Zhao
WB

Mol Cell

DNA Structure-Specific Cleavage of DNA-Protein Crosslinks by the SPRTN Protease.

Authors - Hannah K Reinking
IF

Sci Adv

The impact of C-tactile low-threshold mechanoreceptors on affective touch and social interactions in mice.

Authors - Damien Huzard