Validation Data Gallery

  • Dilution series of EGFP in HEK 293T cell lysate 
Primary antibody: [3H9] 1:1,000
Secondary antibody: goat anti-rat Alexa Fluor® 488 1:1,000

    Dilution series of EGFP in HEK 293T cell lysate Primary antibody: [3H9] 1:1,000 Secondary antibody: goat anti-rat Alexa Fluor® 488 1:1,000

ChromoTek rat anti GFP Monoclonal antibody (3H9)

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins
Cat No. 3h9

Host/IsoType

Rat/IgG2a

Specificity

GFP and GFP derivates

Applications

WB

Conjugate

Unconjugated

Clone No.

3H9

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

Size/Concentration: 

-/ -


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Product Information

Rat monoclonal antibody [3H9] to Green Fluorescent Proteins

DescriptionGFP rat monoclonal antibody [3H9] for WB
ApplicationsWB
Specificity/TargetThe antibody recognizes green fluorescent proteins derived from Aequorea victoria GFP, such as CFP, EGFP, wtGFP, YFP, GFP.
For the complete list, please click here: Fluorescent protein specificity table antibodies
Recommended DilutionWestern Blot: 1:1,000
Note: GFP antibody [3H9] does not work in immunofluorescence.
Host/IsoTypeRat/IgG2a
ClassMonoclonal
TypePrimary Antibody
ImmunogenGreen Fluorescent Protein (GFP)
UniProtKBP42212
Clone No.3H9
ConjugateUnconjugated
FormLiquid
Purification MethodProtein A or G purified
RRIDAB_10773374
Storage BufferPBS, 50% glycerol, preservative: 0.09% sodium azide.
Storage ConditionShipped on ice. Store at -20°C/-4°F. Stable for 1 year after shipment.
Size20 μL; 150 μL
BackgroundGreen Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a rat monoclonal antibody against GFP, reactive to wtGFP, EGFP, YFP, and CFP.

Publications

ApplicationTitle
WB

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler
WB

Nature

TMK-based cell-surface auxin signalling activates cell-wall acidification.

Authors - Wenwei Lin
IP

Cell

A family of conserved bacterial virulence factors dampens interferon responses by blocking calcium signaling.

Authors - Noémie Alphonse
WB

Cell

Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.

Authors - Moumita Chaki
IF

Cell

The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging by Regulation of Insulin Receptor Turnover.

Authors - Riga Tawo
WB

Nat Methods

A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells.

Authors - Yu Chen