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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GFM1 Polyclonal antibody (14274-1-AP)

    WB analysis using 14274-1-AP (same clone as 14274-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14274-1-AP (GFM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GFM1 Polyclonal antibody (14274-1-AP)

    IP experiment of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    IP result of anti-GFM1 (IP:14274-1-AP, 4ug; Detection:14274-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GFM1 Polyclonal antibody (14274-1-AP)

    IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GFM1 Polyclonal antibody (14274-1-AP)

    IHC staining of human kidney using 14274-1-AP (same clone as 14274-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14274-1-AP (GFM1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GFM1 Polyclonal antibody (14274-1-AP)

    IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GFM1 Polyclonal antibody (14274-1-AP)

    IF Staining of HeLa using 14274-1-AP (same clone as 14274-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GFM1 antibody (14274-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 14274-1-PBS in a different storage buffer formulation.

GFM1 Polyclonal antibody, PBS Only

GFM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14274-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

COXPD1, EF Gmt, EFG1, EFGM, EF-Gmt

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14274-1-PBS targets GFM1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5616

Product name: Recombinant human GFM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 400-751 aa of BC049210

Sequence: RMHADMMEDVEEVYAGDICALFGIDCASGDTFTDKANSGLSMESIHVPDPVISIAMKPSNKNDLEKFSKGIGRFTREDPTFKVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN

相同性解析による交差性が予測される生物種
Full Name G elongation factor, mitochondrial 1
Calculated molecular weight 86 kDa
Observed molecular weight85 kDa
GenBank accession numberBC049210
Gene Symbol GFM1
Gene ID (NCBI) 85476
RRIDAB_2110140
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RP9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Different factors catalyze the three stages of protein translation: initiation, elongation, and termination. There are two translational systems in eukaryotes, one in the cytoplasm and the other in the mitochondria. In mitochondria, the elongation phase requires three elongation factors (EF): Tu (TUFM), Ts (TSFM), and G (GFM1)[PMID:19716793]. GFM1 catalyzes translocation during peptide elongation and mediates ribosomal disassembly during ribosome recycling in concert with the ribosomal recycling factor (RRF). [PMID:16487710]

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