GEN1 Monoclonal antibody
GEN1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A10D10
Cat no : 68060-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | HEK-293 cells, U2OS cells, A549 cells, K-562 cells, Daudi cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Sample-dependent, check data in validation data gallery | |
Product Information
68060-1-Ig targets GEN1 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | GEN1 fusion protein Ag31247 相同性解析による交差性が予測される生物種 |
| Full Name | Gen homolog 1, endonuclease (Drosophila) |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 98-100 kDa |
| GenBank accession number | NM_182625 |
| Gene symbol | GEN1 |
| Gene ID (NCBI) | 348654 |
| RRID | AB_2918801 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
GEN1 is a endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. GEN1 efficiently cleaves both single and double HJs contained within large recombination intermediates.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GEN1 antibody 68060-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
