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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GEMIN5 Polyclonal antibody (24897-1-AP)

    WB analysis using 24897-1-AP (same clone as 24897-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24897-1-AP (GEMIN5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GEMIN5 Polyclonal antibody (24897-1-AP)

    WB analysis of HEK-293 using 24897-1-AP (same clone as 24897-1-PBS)

    WB result of GEMIN5 antibody (24897-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GEMIN5 transfected HEK-293 cells. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using GEMIN5 Polyclonal antibody (24897-1-AP)

    IP experiment of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    IP result of anti-GEMIN5 (IP:24897-1-AP, 4ug; Detection:24897-1-AP 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GEMIN5 Polyclonal antibody (24897-1-AP)

    IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GEMIN5 Polyclonal antibody (24897-1-AP)

    IHC staining of mouse cerebellum using 24897-1-AP (same clone as 24897-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 24897-1-AP (GEMIN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GEMIN5 Polyclonal antibody (24897-1-AP)

    IF Staining of HeLa using 24897-1-AP (same clone as 24897-1-PBS)

    Immunofluorescent analysis of HeLa cells using 24897-1-AP (GEMIN5 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24897-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GEMIN5 Polyclonal antibody, PBS Only

GEMIN5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24897-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Gem associated protein 5, Gem-associated protein 5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24897-1-PBS targets GEMIN5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19268

Product name: Recombinant human GEMIN5 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1162-1508 aa of BC113614

Sequence: AVWKSIFSLDTPEQYQEAFQKLQNIKYPSATNNTPAKQLLLHICHDLTLAVLSQQMASWDEAVQALLRAVVRSYDSGSFTIMQEVYSAFLPDGCDHLRDKLGDHQSPATPAFKSLEAFFLYGRLYEFWWSLSRPCPNSSVWVRAGHRTLSVEPSQQLDTASTEETDPETSQPEPNRPSELDLRLTEEGERMLSTFKELFSEKHASLQNSQRTVAEVQETLAEMIRQHQKSQLCKSTANGPDKNEPEVEAEQPLCSSQSQCKEEKNEPLSLPELTKRLTEANQRMAKFPESIKAWPFPDVLECCLVLLLIRSHFPGCLAQEMQQQAQELLQKYGNTKTYRRHCQTFCM

相同性解析による交差性が予測される生物種
Full Name gem (nuclear organelle) associated protein 5
Calculated molecular weight 1508 aa, 169 kDa
Observed molecular weight 168 kDa
GenBank accession numberBC113614
Gene Symbol GEMIN5
Gene ID (NCBI) 25929
RRIDAB_2879784
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEQ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GEMIN5 also named as Gem associated protein 5 is a 1508 amino-acid protein, which belongs to the WD repeat gemin-5 family. The molecular weight of GEMIN5 is 168 kDa GEMIN5 is a component of SMN complex, which plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. GEMIN5 is identified as the factor that allows the SMN complex to distinguish snRNAs from other cellular RNAs for snRNP biogenesis. GEMIN5 is found in nucleoplasm, nuclear bodies and cytoplasm.

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