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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GEF-H1 Polyclonal antibody (24472-1-AP)

    WB analysis using 24472-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24472-1-AP (GEF-H1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GEF-H1 Polyclonal antibody (24472-1-AP)

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using GEF-H1 Polyclonal antibody (24472-1-AP)

    IHC staining of human breast cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GEF-H1 Polyclonal antibody (24472-1-AP)

    IHC staining of human stomach cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GEF-H1 Polyclonal antibody (24472-1-AP)

    IHC staining of human stomach cancer using 24472-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24472-1-AP (GEF-H1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GEF-H1 Polyclonal antibody (24472-1-AP)

    IF Staining of HepG2 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using GEF-H1 Polyclonal antibody (24472-1-AP)

    IF Staining of MCF-7 using 24472-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GEF-H1 Polyclonal antibody (24472-1-AP)

    IF Staining of HeLa using 24472-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GEF-H1 antibody (24472-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293T cells using GEF-H1 Polyclonal antibody (24472-1-AP)

    FC experiment of HEK-293T using 24472-1-AP

    1X10^6 HEK-293T cells were intracellularly stained with 0.4 ug Anti-Human GEF-H1 (24472-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

GEF-H1 Polyclonal antibody

GEF-H1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 24472-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ARHGEF2, LFP40, KIAA0651, GEFH1, GEF H1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, HEK-293T cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, MCF-7 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24472-1-AP targets GEF-H1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13352

Product name: Recombinant human GEF-H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 610-959 aa of BC020567

Sequence: PRGLFRSESLESPRGERLLQDAIREVEGLKDLLVGPGVELLLTPREPALPLEPDSGGNTSPGVTANGEARTFNGSIELCRADSDSSQRDRNGNQLRSPQEEALQRLVNLYGLLHGLQAAVAQQDTLMEARFPEGPERREKLCRANSRDGEAGRAGAAPVAPEKQATELALLQRQHALLQEELRRCRRLGEERATEAGSLEARLRESEQARALLEREAEEARRQLAALGQTEPLPAEAPWARRPVDPRRRSLPAGDALYLSFNPPQPSRGTDRLDLPVTTRSVHRNFEDRERQELGSPEERLQDSSDPDTGSEEEGSSRLSPPHSPRDFTRMQDIPEETESRDGEAVASES

相同性解析による交差性が予測される生物種
Full Name rho/rac guanine nucleotide exchange factor (GEF) 2
Calculated molecular weight 112 kDa
Observed molecular weight 111 kDa
GenBank accession numberBC020567
Gene Symbol GEF-H1/ARHGEF2
Gene ID (NCBI) 9181
RRIDAB_2879560
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92974
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for GEF-H1 antibody 24472-1-APDownload protocol
IHC protocol for GEF-H1 antibody 24472-1-APDownload protocol
IF protocol for GEF-H1 antibody 24472-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Rep

Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.

Authors - J J David Ho
View Article
human,mouseWB,IP,CoIP

Cell Death Dis

Septin11 promotes hepatocellular carcinoma cell motility by activating RhoA to regulate cytoskeleton and cell adhesion

Authors - Lisheng Fu
View Article