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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse bladder tissue using GDPD3 Polyclonal antibody (20390-1-AP)

    IHC staining of mouse bladder using 20390-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 20390-1-AP (GDPD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse bladder tissue using GDPD3 Polyclonal antibody (20390-1-AP)

    IHC staining of mouse bladder using 20390-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 20390-1-AP (GDPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GDPD3 Polyclonal antibody

GDPD3 Polyclonal Antibody for IHC, ELISA
Cat No. 20390-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

GDE7, Glycerophosphodiester phosphodiesterase 7, Glycerophosphodiester phosphodiesterase domain-containing protein 3, Lysophospholipase D GDPD3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inmouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20390-1-AP targets GDPD3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14230

Product name: Recombinant human GDPD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-260 aa of BC002714

Sequence: MENSMAQRSDLLELDCQLTRDRVVVVSHDENLCRQSGLNRDVGSLDFEDLPLYKEKLEVYFSPGHFAHGSDRRMVRLEDLFQRFPRTPMSVEIKGKNEELIREIAGLVRRYDRNEITIWASEKSSVMKKCKAANPEMPLSFTISRGFWVLLSYYLGLLPFIPIPEKFFFCFLPNIINRTYFPFSCSCLNQLLAVVSKWLIMRKSLIRHLEERGVQVVFWCLNEESDFEAAFSVGATGVITDYPTALRHYLDNHGPAARTS

相同性解析による交差性が予測される生物種
Full Name glycerophosphodiester phosphodiesterase domain containing 3
Calculated molecular weight 318 aa, 37 kDa
GenBank accession numberBC002714
Gene Symbol GDPD3
Gene ID (NCBI) 79153
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L5L3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDPD3 (glycerophosphodiester phosphodiesterase domain-containing 3), also termed GDE7, encodes a multi-pass membrane protein with lysophospholipase D activity. It hydrolyzes lysophospholipids to generate lysophosphatidic acid (LPA), a key lipid mediator involved in glycerol phosphate pathway-mediated triacylglycerol synthesis and hepatic steatosis. (PMID: 32430316)

Protocols

Product Specific Protocols
IHC protocol for GDPD3 antibody 20390-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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