Validation Data Gallery
Tested Applications
| Positive WB detected in | HUVEC cells, SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33521-1-AP targets GDF6/BMP-13 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag40394 Product name: Recombinant human BMP-13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-455 aa of NM_001001557 Sequence: TAFASRHGKRHGKKSRLRCSKKPLHVNFKELGWDDWIIAPLEYEAYHCEGVCDFPLRSHLEPTNHAIIQTLMNSMDPGSTPPSCCVPTKLTPISILYIDAGNNVVYKQYEDMVVESCGCR 相同性解析による交差性が予測される生物種 |
| Full Name | growth differentiation factor 6 |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 40-50 kDa |
| GenBank accession number | NM_001001557 |
| Gene Symbol | GDF6 |
| Gene ID (NCBI) | 392255 |
| RRID | AB_3743000 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6KF10 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GDF6, also known as BMP-13, is a member of the transforming growth factor-beta superfamily. GDF6 is a multifunctional growth factor that mainly functions in cells derived from the mesoderm. It can regulate the proliferation and differentiation of the retina and bones(PMID: 23307924). Mutations in GDF6 are associated with Klippel-Feil syndrome(PMID: 18425797).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GDF6/BMP-13 antibody 33521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |