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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using GDE1 Polyclonal antibody (11272-2-AP)

    WB analysis of HEK-293 using 11272-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11272-2-AP (GDE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GDE1 Polyclonal antibody (11272-2-AP)

    WB analysis of PC-3 using 11272-2-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11272-2-AP (GDE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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GDE1 Polyclonal antibody

GDE1 Polyclonal Antibody for ELISA
Cat No. 11272-2-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

363E6.2, GDE1, MIR16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11272-2-AP targets GDE1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GDE1 fusion protein Ag1821 相同性解析による交差性が予測される生物種
Full Name glycerophosphodiester phosphodiesterase 1
Calculated molecular weight 38 kDa
Observed molecular weight 55-66 kDa
GenBank accession numberBC014981
Gene Symbol GDE1
Gene ID (NCBI) 51573
RRIDAB_2247628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GDE1(glycerophosphodiester phosphodiesterase 1) is also named as MIR16 and belongs to the glycerophosphoryl diester phosphodiesterase family. It is an 43 kDa integral membrane glycoprotein, which remains associated with membranes after alkaline (pH 11) extraction and is sensitive to endoglycosidase H and most likely involved in the remodeling of membrane PIs and regulation of membrane phospholipid composition, and hence in intracellular membrane trafficking, as well as in the modulation of G proteinmediated signaling(PMID:12576545). The full length protein has two glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for GDE1 antibody 11272-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Invest Ophthalmol Vis Sci

Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.

Authors - Sasha M Milbeck
View Article