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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using GDAP1L1 Polyclonal antibody (11977-1-AP)

    WB analysis of mouse brain using 11977-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using GDAP1L1 Polyclonal antibody (11977-1-AP)

    WB analysis of fetal human brain using 11977-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using GDAP1L1 Polyclonal antibody (11977-1-AP)

    WB analysis of Y79 using 11977-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11977-1-AP (GDAP1L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GDAP1L1 Polyclonal antibody (11977-1-AP)

    IHC staining of human gliomas using 11977-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11977-1-AP (GDAP1L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using GDAP1L1 Polyclonal antibody (11977-1-AP)

    IHC staining of human gliomas using 11977-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11977-1-AP (GDAP1L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GDAP1L1 Polyclonal antibody

GDAP1L1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11977-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Synonyms

GDAP1 L1, GDAP1L1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, Y79 cells, fetal human brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11977-1-AP targets GDAP1L1 in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2602

Product name: Recombinant human GDAP1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-367 aa of BC009014

Sequence: MATPNNLTPTNCSWWPISALESDAAKPAEAPDAPEAASPAHWPRESLVLYHWTQSFSSQKVRLVIAEKGLVCEERDVSLPQSEHKEPWFMRLNLGEEVPVIIHRDNIISDYDQIIDYVERTFTGEHVVALMPEVGSLQHARVLQYRELLDALPMDAYTHGCILHPELTTDSMIPKYATAEIRRHLANATTDLMKLDHEEEPQLSEPYLSKQKKLMAKILEHDDVSYLKKILGELAMVLDQIEAELEKRKLENEGQKCELWLCGCAFTLADVLLGATLHRLKFLGLSKKYWEDGSRPNLQSFFERVQRRFAFRKVLGDIHTTLLSAVIPNAFRLVKRKPPSFFGASFLMGSLGGMGYFAYWYLKKKYI

相同性解析による交差性が予測される生物種
Full Name ganglioside-induced differentiation-associated protein 1-like 1
Calculated molecular weight 367 aa, 42 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC009014
Gene Symbol GDAP1L1
Gene ID (NCBI) 78997
RRIDAB_2232105
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MZ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for GDAP1L1 antibody 11977-1-APDownload protocol
WB protocol for GDAP1L1 antibody 11977-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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