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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCOM1 Polyclonal antibody (18129-1-AP)

    WB analysis using 18129-1-AP (same clone as 18129-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18129-1-AP (GCOM1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCOM1 Polyclonal antibody (18129-1-AP)

    IHC staining of human liver cancer using 18129-1-AP (same clone as 18129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18129-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GCOM1 Polyclonal antibody (18129-1-AP)

    IHC staining of human liver cancer using 18129-1-AP (same clone as 18129-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18129-1-AP (GCOM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18129-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GCOM1 Polyclonal antibody (18129-1-AP)

    IF Staining of HepG2 using 18129-1-AP (same clone as 18129-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using GCOM1 antibody 18129-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18129-1-PBS in a different storage buffer formulation.

GCOM1 Polyclonal antibody, PBS Only

GCOM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18129-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18129-1-PBS targets GCOM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12708

Product name: Recombinant human GCOM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 118-466 aa of BC101645

Sequence: MYGDYDEMRQKIRQLTQELSVSHAQQEYLENHIQTQSSALDRFNAMNSALASDSIGLQKTLVDVTLENSNIKDQIRNLQQTYEASMDKLREKQRQLEVAQVENQLLKMKVESSQEANAEVMREMTKKLYSQYEEKLQEEQRKHSAEKEALLEETNSFLKAIEEANKKMQAAEISLEEKDQRIGELDRLIERMEKERHQLQLQLLEHETEMSGELTDSDKERYQQLEEASASLRERIRHLDDMVHCQQKKVKQMVEEIESLKKKLQQKQLLILQLLEKISFLEGENNELQSRLDYLTETQAKTEVETREIGVGCDLLPSQTGRTREIVMPSRNYTPYTRVLELTMKKTLT

相同性解析による交差性が予測される生物種
Full Name GRINL1A complex locus
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 64 kDa, 44-54 kDa
GenBank accession numberBC101645
Gene Symbol GCOM1
Gene ID (NCBI) 145781
RRIDAB_2232101
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CAP1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GCOM1, also named as Myozap, Gup and Gcom, belongs to the GCOM1 family. It plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF. GCOM1 has 11 isoforms with MW 44-54kd and 64kd.

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