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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GCNT3 Polyclonal antibody (17101-1-AP)

    WB analysis using 17101-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17101-1-AP (GCNT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using GCNT3 Polyclonal antibody (17101-1-AP)

    IHC staining of human stomach cancer using 17101-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17101-1-AP (GCNT3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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GCNT3 Polyclonal antibody

GCNT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17101-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2/4GnT, C2GnT mucin type, C2GnT-M, C2GnT-mucin type

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17101-1-AP targets GCNT3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCNT3 fusion protein Ag10765 相同性解析による交差性が予測される生物種
Full Name glucosaminyl (N-acetyl) transferase 3, mucin type
Calculated molecular weight 438 aa, 51 kDa
Observed molecular weight51 kDa
GenBank accession numberBC017032
Gene Symbol GCNT3
Gene ID (NCBI) 9245
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95395
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mucin-type core 2 1,6-N-acetylglucosaminyltransferase enzyme (C2GnT-M), encoded by the GCNT3 gene, has been reported to be altered in tumours and to possess tumour suppressor properties.GCNT3 is markedly expressed in both lung cancer tissues and BRCA tissues, and high GCNT3 levels correlate with poorer outcomes in patients undergoing radiotherapy (PMID: 25466507, 39697709). Elevated levels of GCNT3 promoted cell growth, migration, cell cycle development, and invasion, in addition to EMT, while suppressing apoptosis (PMID: 38789846). It is best to use unboiled samples for detection, for membrane proteins are hydrophobic and tend to aggregate when boiled at high temperatures.

Protocols

Product Specific Protocols
WB protocol for GCNT3 antibody 17101-1-APDownload protocol
IHC protocol for GCNT3 antibody 17101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols