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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using GCNF Polyclonal antibody (12712-1-AP)

    WB analysis of PC-3 using 12712-1-AP (same clone as 12712-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using GCNF Polyclonal antibody (12712-1-AP)

    WB analysis of mouse testis using 12712-1-AP (same clone as 12712-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using GCNF Polyclonal antibody (12712-1-AP)

    WB analysis of K-562 using 12712-1-AP (same clone as 12712-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 12712-1-AP (GCNF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using GCNF Polyclonal antibody (12712-1-AP)

    IP experiment of mouse testis using 12712-1-AP (same clone as 12712-1-PBS)

    IP result of anti-GCNF (IP:12712-1-AP, 4ug; Detection:12712-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GCNF Polyclonal antibody (12712-1-AP)

    IHC staining of human testis using 12712-1-AP (same clone as 12712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using GCNF Polyclonal antibody (12712-1-AP)

    IHC staining of human testis using 12712-1-AP (same clone as 12712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12712-1-AP (GCNF antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12712-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

GCNF Polyclonal antibody, PBS Only

GCNF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12712-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12712-1-PBS targets GCNF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3395

Product name: Recombinant human NR6A1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC030600

Sequence: MERDEPPPSGGGGGGGSAGFLEPPAALPPPPRNGFCQDELAELDPGTNDRAEQRTCLICGDRATGLHYGIISCEGCKGFFKRSICNKRVYRCSRDKNCVMSRKQRNRCQYCRLLKCLQMGMNRKAIREDGMPGGRNKSIGPVQISEEEIERIMSGQEFEEEANHWSNHGDSDHSSPGNRASESNQPSPGSTLSSRSVELNGFMAFREQYMGMSVPPHYQYIPHLFSYSGHSPLLPQQARSLDPQSYSLIHQLLSAEDLEPLGTPMLIEDGYAVTQAELFALLCRLADELLFRQIAWIKKLPFFCELSIKDYTCLLSSTWQELILLSSLTVYSKQIFGELADVTAKYSPSD

相同性解析による交差性が予測される生物種
Full Name nuclear receptor subfamily 6, group A, member 1
Calculated molecular weight 475 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC030600
Gene Symbol GCNF
Gene ID (NCBI) 2649
RRIDAB_10640439
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15406
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

NR6A1 (Nuclear Receptor Subfamily 6 Group A member), also known as GCNF (Germ Cell Nuclear Factor), is an orphan member of the nuclear receptor gene superfamily. It has been shown to be expressed in the nervous system during development and during specific stages in maturing germ cells of the ovary and testis in the adult, and has probable roles in gametogenesis, neurogenesis, and normal embryonic development during gastrulation. Inactivation of GCNF in mouse results in abnormal posterior development, impaired midbrain development, insufficient closure of the neural tube, and eventual embryonic death. GCNF has been shown to be a repressor of OCT-4 and plays a critical role in the control of gene expression during embryogenesis and spermatogenesis. This antibody specifically reacts with the 55kd NR6A1 protein.

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