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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GBP3 Polyclonal antibody (30719-1-AP)

    WB analysis using 30719-1-AP (same clone as 30719-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30719-1-AP (GBP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse cerebellum using 30719-1-AP (same clone as 30719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse cerebellum using 30719-1-AP (same clone as 30719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse lung using 30719-1-AP (same clone as 30719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using GBP3 Polyclonal antibody (30719-1-AP)

    IHC staining of mouse lung using 30719-1-AP (same clone as 30719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 30719-1-AP (GBP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30719-1-PBS in a different storage buffer formulation.

GBP3 Polyclonal antibody, PBS Only

GBP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30719-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30719-1-PBS targets GBP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag33889

Product name: Recombinant human GBP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 535-595 aa of BC140837

Sequence: RERAQLLEEQEKTLTSKLQEQARVLKERCQGESTQLQNEIQKLQKTLKKKTKRYMSHKLKI*

相同性解析による交差性が予測される生物種
Full Name guanylate binding protein 3
Observed molecular weight68 kDa
GenBank accession numberBC140837
Gene Symbol GBP3
Gene ID (NCBI) 2635
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0R5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Guanylate-binding protein 3 (GBP3) is involved in the proliferation of glioma cells through regulating SQSTM1-ERK1/2 pathway. GBP3 has 2 isoforms, one is 595 amino acids long at 68 kDa and the other is at nearly 62 kDa.

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