Validation Data Gallery

Filter: AllWBIHC

WB analysis using 27299-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27299-1-AP (GBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 27299-1-AP
WB result of GBP2 antibody (27299-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBP2 transfected HeLa cells.
WB analysis of HeLa using 27299-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 27299-1-AP (GBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 27299-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human spleen using 27299-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27299-1-AP (GBP2 Antibody) at dilution of 1:200 (under 40x lens).

Featured Product
KD/KO Validated

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27299-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

GBP 2, GBP2, GTP binding protein 2, HuGBP 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A375 cells, HeLa cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected in	human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 5 publications below

Product Information

27299-1-AP targets GBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25433 Product name: Recombinant human GBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 435-481 aa of BC022272 Sequence: TQKLQELKNKYYQVPRKGIQAKEVLKKYLESKEDVADALLQTDQSLS 相同性解析による交差性が予測される生物種
Full Name	guanylate binding protein 2, interferon-inducible
Calculated molecular weight	591 aa, 67 kDa
Observed molecular weight	67 kDa
GenBank accession number	BC022272
Gene Symbol	GBP2
Gene ID (NCBI)	2634
RRID	AB_2880836
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P32456
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for GBP2 antibody 27299-1-AP	Download protocol
WB protocol for GBP2 antibody 27299-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Immunol IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death. Authors - Rajendra Karki View Article
human	WB,IHC	Anticancer Res Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma. Authors - Qingyuan Liu KD Validated View Article
human	WB,IHC	Oncol Rep GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling Authors - Shujiang Ye View Article
human,mouse	WB,IHC	Microvasc Res GBP2 inhibits pathological angiogenesis in the retina via the AKT/mTOR/VEGFA axis Authors - Xiaoxiang Xu KD Validated View Article
human	WB,IHC	Cancer Sci Crosstalk between GBP2 and M2 macrophage promotes the ccRCC progression Authors - Wei Zheng KO Validated View Article
mouse	WB,IHC	Adv Sci (Weinh) CpG-Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2-Mediated Remodeling of Tumor-Associated Macrophages Authors - Jiaqiang Xiong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 27299-1-AP

WB analysis of HeLa using 27299-1-AP

WB analysis of HeLa using 27299-1-AP

IHC staining of human spleen using 27299-1-AP

IHC staining of human spleen using 27299-1-AP

GBP2 Polyclonal antibody

GBP2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

IRF8 Regulates Gram-Negative Bacteria-Mediated NLRP3 Inflammasome Activation and Cell Death.

Guanylate-binding Protein 2 Expression Is Associated With Poor Survival and Malignancy in Clear-cell Renal Cell Carcinoma.

GBP2 promotes clear cell renal cell carcinoma progression through immune infiltration and regulation of PD‑L1 expression via STAT1 signaling

GBP2 inhibits pathological angiogenesis in the retina via the AKT/mTOR/VEGFA axis

Crosstalk between GBP2 and M2 macrophage promotes the ccRCC progression

CpG-Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2-Mediated Remodeling of Tumor-Associated Macrophages