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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

GBA Polyclonal antibody

GBA Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20622-1-AP

Synonyms

GBA, GBAP

Validation Data Gallery

Applications:
  • IHC staining of human breast cancer using 20622-1-AP

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 20622-1-AP

    IHC staining of human breast cancer using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 20622-1-AP

    IHC staining of human brain using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 20622-1-AP

    IHC staining of human brain using 20622-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 20622-1-AP (GBA antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 20622-1-AP

    IF Staining of SH-SY5Y using 20622-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 20622-1-AP (GBA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20622-1-AP is GBA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBA fusion protein Ag13981 相同性解析による交差性が予測される生物種
Full Name glucosidase, beta; acid, pseudogene
Calculated molecular weight 321 aa, 36 kDa
GenBank accession numberBC000349
Gene symbol GBAP
Gene ID (NCBI) 2630
RRIDAB_10696317
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for GBA antibody 20622-1-APDownload protocol
IF protocol for GBA antibody 20622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols