Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, HeLa cells, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
17164-1-AP targets GBA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10606 Product name: Recombinant human GBA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 574-927 aa of BC011363 Sequence: RRYLMSGVMAPVKRRNVIPHDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVMESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQDKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEVFPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATMIQEGLTWEGFQTAEGCYRTVWERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLALQQQQHKKASWPKVKQGTGLRTGPMFGPKEAMANLSPE 相同性解析による交差性が予測される生物種 |
| Full Name | glucosidase, beta (bile acid) 2 |
| Calculated molecular weight | 927 aa, 105 kDa |
| Observed molecular weight | 105 kDa |
| GenBank accession number | BC011363 |
| Gene Symbol | GBA2 |
| Gene ID (NCBI) | 57704 |
| RRID | AB_2109077 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HCG7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
GBA2(Non-lysosomal glucosylceramidase) is also named as KIAA1605,NLGase and belongs to the non-lysosomal glucosylceramidase family.It catalyzes the conversion of glucosylceramide to free glucose and ceramide and is involved in sphingomyelin generation and prevention of glycolipid accumulation.GBA2 contains a putative membrane-spanning domain and 2 potential N-glycosylation sites. However, glycosidase treatment indicated that GBA2 is not a glycoprotein(PMID:11489889).It has 3 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for GBA2 antibody 17164-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |