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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis of A549 using 68405-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using GATC Monoclonal antibody (68405-1-Ig)

    WB analysis using 68405-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68405-1-Ig (GATC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using GATC Monoclonal antibody (68405-1-Ig)

    IF Staining of HEK-293 using 68405-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using GATC antibody (68405-1-Ig, Clone: 2F9C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Flow cytometry (FC) experiment of HEK-293 cells using GATC Monoclonal antibody (68405-1-Ig)

    FC experiment of HEK-293 using 68405-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug GATC Monoclonal antibody (68405-1-Ig, Clone:2F9C8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

GATC Monoclonal antibody

GATC Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68405-1-Ig
Clone No.2F9C8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

15E1.2, EC:6.3.5.-, GatC like protein, Glu-AdT subunit C, Glutamyl-tRNA(Gln) amidotransferase subunit C, mitochondrial

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HEK-293 cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68405-1-Ig targets GATC in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag19857

Product name: Recombinant human GATC protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-136 aa of BC107145

Sequence: MWSRLVWLGLRAPLGGRQGFTSKADPQGSGRITAAVIEHLERLALVDFGSREAVARLEKAIAFADRLRAVDTDGVEPMESVLEDRCLYLRSDNVVEGNCADELLQNSHRVVEEYFVAPPGNISLPKLDEQEPFPHS

相同性解析による交差性が予測される生物種
Full Name glutamyl-tRNA(Gln) amidotransferase, subunit C homolog (bacterial)
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC107145
Gene Symbol GATC
Gene ID (NCBI) 283459
RRIDAB_3085122
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO43716
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

GATC encodes a subunit of the glutamyl-tRNA(Gln) amidotransferase protein complex (GatCAB), which plays a role in aminoacylation of mitochondrial glutaminyl mt-tRNA. The complex plays an role in mitochondrial translation and protein synthesis (PubMed: 30283131).

Protocols

Product Specific Protocols
FC protocol for GATC antibody 68405-1-IgDownload protocol
IF protocol for GATC antibody 68405-1-IgDownload protocol
WB protocol for GATC antibody 68405-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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