Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 55433-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55433-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 55433-1-AP
Immunohistochemical analysis of paraffin-embedded human colon using 55433-1-AP (GATA5 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 55433-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA5 antibody (55433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

GATA5 Polyclonal antibody

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 55433-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IF	See 1 publications below
IP	See 2 publications below

Product Information

55433-1-AP targets GATA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	GATA binding protein 5
Calculated molecular weight	41 kDa
Observed molecular weight	41 kDa
GenBank accession number	NM_080473
Gene Symbol	GATA5
Gene ID (NCBI)	140628
RRID	AB_2881330
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWX5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for GATA5 antibody 55433-1-AP	Download protocol
IHC protocol for GATA5 antibody 55433-1-AP	Download protocol
IF protocol for GATA5 antibody 55433-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IP	Cancers (Basel) Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway. Authors - Qinghua Wang View Article
human,mouse	WB,IF,IP	Biochem Biophys Res Commun Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways. Authors - Anmin Ren View Article
human	WB	Nat Commun Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy Authors - Wenjing Bai View Article
mouse	WB	Int J Biol Sci Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice Authors - Qianqian Feng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 55433-1-AP

WB analysis of HepG2 using 55433-1-AP

IHC staining of human colon using 55433-1-AP

IHC staining of human colon using 55433-1-AP

IHC staining of human colon using 55433-1-AP

IHC staining of human colon using 55433-1-AP

IF Staining of HepG2 using 55433-1-AP

GATA5 Polyclonal antibody

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.

Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways.

Defining ortholog-specific UHRF1 inhibition by STELLA for cancer therapy

Cardiovascular Mettl3 Deficiency Causes Congenital Cardiac Defects and Postnatal Lethality in Mice