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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of PC-3 using 68012-1-Ig (same clone as 68012-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of HEK-293 using 68012-1-Ig (same clone as 68012-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of HepG2 using 68012-1-Ig (same clone as 68012-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCCIT cells using GATA4 Monoclonal antibody (68012-1-Ig)

    WB analysis of NCCIT using 68012-1-Ig (same clone as 68012-1-PBS)

    NCCIT cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68012-1-PBS in a different storage buffer formulation.

GATA4 Monoclonal antibody, PBS Only

GATA4 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68012-1-PBS
Clone No.2F5C6

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, Indirect ELISA

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68012-1-PBS targets GATA4 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30392

Product name: Recombinant human GATA4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 260-442 aa of BC101580

Sequence: RLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA

相同性解析による交差性が予測される生物種
Full Name GATA binding protein 4
Calculated molecular weight 442 aa, 45 kDa
Observed molecular weight44,45 kDa
GenBank accession numberBC101580
Gene Symbol GATA4
Gene ID (NCBI) 2626
RRIDAB_2918759
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP43694
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).

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